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A-ProTrap Butyl resin is a chromatography media, can remove aggregates efficiently from antibodies, synthesized by standard polysaccharide ligand modification technology. After affinity chromatography like protein A, protein L or protein G, the antibody samples are passed through A-ProTrap Butyl chromatography, the aggregates are bound to the hydrophobic ligand of the chromatography media and antibody monomers are coming out in flowthrough fraction. A-ProTrap Butyl can be used as flow-through purification mode that selectively bounds such as aggregates or polymers.
Fig 1. OPM analysis of A-ProTrap Butyl resin
Cross-linked spherical A-ProTrap beads are prepared by unique process from natural polysaccharides. Primarily composed of agarobiose repeating units (alternating D-galactose and 3,6-anhydro-L-galactose), finally beads are highly hydrophilic, minimize the non-specific binding. A-ProTrap Butyl, made from crosslinked A-ProTrap beads, benefits from this structure by offering efficient flow velocity for large biomolecules. This helps improve performance when purifying large proteins and other biological substances.
Partial structure of A-ProTrap Butyl
Ligand structure for A-ProTrap Butyl media is described.
A-ProTrap Butyl enable high-flow operations, for efficient purification in biopharmaceutical industries high flow operation is essential. The figures below show pressure-flow velocity curves of A-ProTrap Butyl. A-ProTrap Butyl resin is operable at practical flow velocities and pressures.
A-ProTrap Butyl have high efficiency in mass transfer and excellent Dynamic Binding Capacities, particularly for large biomolecules like Immunoglobulins (IgG) also for HSA and Ribonuclease. Because of these special qualities, A-ProTrap Butyl media can be used in downstream processes in the purification of biopharmaceuticals
| Characteristics | |
|---|---|
| Ligand | Butyl group |
| Type | Hydrophobic |
| Base Matrix | Highly Cross-linked agarose Beads |
| Particle Size (µm) | 40–130 µm (Avg: 90 µm) |
| pH Working Range | 2–14 |
| Operating Pressure | Up to 2 bar (0.2 MPa) |
| Chemical Stability | 0.5 N NaOH |
| Dynamic Binding Capacity (mg/mL resin) |
HSA*: 30 Human IgG**: 10 |
| Supplied | Suspension in 20% Ethanol |
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