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A-ProTrap SP is a strong cation exchanger chromatography media based on spherical particles manufactured from crosslinked Agarose. Each offers excellent flow properties, mechanical stability, and chemical resistance. In this ion exchange resin, the sulphopropyl (SP) functional group is attached to the Agarose beads, which remains charged and maintains consistently high capacity over the entire working range, pH 3 to 11. These ion exchangers are ideally suited for both laboratory and process scale chromatography of proteins, peptides, and other biomolecules.
Optical micrograph of A-ProTrap SP Resin
Cross-linked spherical agarose beads are prepared by unique process from natural polysaccharides. Primarily composed of agarobiose repeating units (alternating D-galactose and 3,6-anhydro-L-galactose), finally beads are highly hydrophilic, minimize the non-specific binding. A-ProTrap SP, made from cross-linked agarose beads, benefits from this structure by offering efficient flow velocity for large biomolecules. This helps improve performance when purifying large proteins and other biological substances.
A-ProTrap SP have high efficiency in mass transfer and excellent Dynamic Binding Capacities, particularly for large biomolecules like Immunoglobulins (IgG) also for HNA (Human Normal Albumin) and Lysozyme, Ribonuclease.
Because of these special qualities, A-ProTrap SP media can be used in downstream processes in the purification of biopharmaceuticals. With good reliability, protein separation has been scaled up using A-ProTrap SP from laboratory size and no discernible variations were seen in the purity of single peak at the time of protein separation.
| Characteristics | |
|---|---|
| Ion Exchange Type (Functional Group) | Sulphopropyl group |
| Base Matrix | Spherical, Cross-linked Agarose Beads |
| Particle Size (µm) | 30 µm to 165 µm |
| pH Working Range | 2–12 |
| Operating Pressure | Up to 2 bar (0.2 Mpa) |
| Ion Exchange Capacity (meq / ml-gel) | 0.11–0.20 |
| Dynamic Binding Capacity (mg/mL) |
human-γ-globulin (IVIG): ≥50 Lysozyme: 90 |
| Supplied | Suspension in 20% Ethanol |
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