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C-ProTrap Q strong anion exchanger is a surface-modified, highly cross-linked medium with dextran scaffold structure. It delivers high dynamic binding capabilities and maintains stability even at elevated flow rates. The optimized media's excellent performance presents a substantial chance to boost the throughput of downstream purification. In this ion exchange resin, the Quaternary amine (Glycidyl trimethyl ammonium chloride) functional group is attached to the cellulose beads, which remains charged and maintains consistently high capacity over the entire working range, pH 2 to 11.
Optical micrograph of C-ProTrap Q Resin
Cellulose is a natural polysaccharide with a unique crystalline structure, unlike non- crystalline polysaccharides like agarose. This gives cellulose a special pore structure, which plays an important role in chromatography. C-ProTrap Q, made from crosslinked cellulose and scaffold with Dextran, benefits from this structure by offering good mechanical strength and efficient flow of large biomolecules. This helps improve performance when purifying large proteins and other biological substances even with high flow rates.
C-ProTrap Q have high efficiency in mass transfer and excellent Dynamic Binding Capacities, particularly for HSA), and large biomolecules like Immunoglobulins (IgG).
Because of these special qualities, C-ProTrap Q media can be used in downstream processes in the purification of biopharmaceuticals. With good reliability, protein separation has been scaled up using C-ProTrap Q from laboratory size and no discernible variations were seen in the purity of single peak at the time of protein separation.
| Attribute | C-ProTrap Q Resin |
|---|---|
| Ion Exchange Type (Functional Group) | Trimethylaminoethyl group |
| Type | Strong anion |
| Base Matrix | Spherical, Highly Cross-linked Cellulose Beads with Dextran Scaffold |
| Particle Size (μm) | 40-130 µm |
| Exclusion Limit (Da) | 360,000 |
| pH Working Range | 2 to 11 |
| Operating Pressure | Up to 2 bar (0.2 MPa) |
| Ion Exchange Capacity (meq/ml-gel) | 0.1-0.24 |
| Chemical Stability | 0.5N NaOH |
| Dynamic Binding Capacity (mg/ml) | BSA 45 Human IgG 35 |
| Supplied | Suspension in 20% Ethanol |
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