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DC-ProTrap SP is a strong cation exchanger chromatography media based on spherical particles manufactured from crosslinked cellulose. Each offers excellent flow properties, mechanical stability, and chemical resistance. In this ion exchange resin, the sulphopropyl (SP) functional group is attached to the cellulose beads, which remains charged and maintains consistently high capacity over the entire working range, pH 3 to 11. These ion exchangers are ideally suited for both laboratory and process scale chromatography of proteins, peptides, and other biomolecules.
Optical micrograph of DC-ProTrap SP Resin
Cellulose is a natural polysaccharide with a unique crystalline structure, unlike non- crystalline polysaccharides like agarose. This gives cellulose a special pore structure, which plays an important role in chromatography. DC-ProTrap SP, made from crosslinked cellulose, benefits from this structure by offering good mechanical strength and efficient flow of large biomolecules. This helps improve performance when purifying large proteins and other biological substances.
DC-ProTrap SP have high efficiency in mass transfer and excellent Dynamic Binding Capacities, particularly for large biomolecules like Immunoglobulins (IgG) also for HNA (Human Normal Albumin) and Lysozyme, Ribonuclease.
Because of these special qualities, DC-ProTrap SP media can be used in downstream processes in the purification of biopharmaceuticals. With good reliability, protein separation has been scaled up using DC-ProTrap SP from laboratory size and no discernible variations were seen in the purity of single peak at the time of protein separation.
| Attribute | DC-ProTrap SP Resin |
|---|---|
| Ion Exchange Type (Functional Group) | Sulphopropyl group |
| Base Matrix | Spherical, Cross-linked Cellulose Beads |
| Particle Size (μm) | 40-130 µm |
| Exclusion Limit (Da) | 360,000 |
| pH Working Range | 2-12 |
| Operating Pressure | Up to 2 bar (0.2 MPa) |
| Ion Exchange Capacity (meq/ml-gel) | 0.11-0.20 |
| Binding Capacity (mg/mL) | Human IgG 93 Lysozyme 195 |
| Supplied | Suspension in 20% Ethanol |
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