ProTrap G is an advanced affinity chromatography resin in which recombinant Protein G (rProtein G) is covalently immobilized on agarose beads. It is widely used in bioprocessing and antibody purification workflows for the efficient and selective isolation of immunoglobulins (IgGs) from various sources, including cell culture supernatants and biological fluids. Its high binding affinity and broad subclass specificity make it ideal for research, diagnostic, and biopharmaceutical applications.
 
       
         
      | Properties | |
|---|---|
| Matrix | Crosslinked agarose beads | 
| Ligand | High molecular weight Dextran sulfate | 
| Particle diameter (µm) | 30–165 µm | 
| Lysozyme binding capacity | ≥20 mg/mL resin | 
| Sulphur content | 50 µM/mL | 
| Recommended CIP solution | 0.2(M) NaOH, 0.2(M) NaOH in 20% EtOH, 0.2(M) NaOH in 95% EtOH | 
| pH stability range | 1–13 | 
| Storage | 2–8 °C in 20% ethanol | 
| Characteristics of Protein Recovery | Possible to enter the pore but low adsorption and high recovery under salt addition conditions | 
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