ProTrap Pro A Resin is a next-generation affinity chromatography resin designed for efficient and high-purity purification of monoclonal and polyclonal antibodies, particularly IgG from various species. Incorporating a genetically engineered recombinant Protein A ligand, this resin offers enhanced alkaline stability and superior dynamic binding capacity compared to traditional Protein A resins. ProTrap Pro A is ideal for both research and bioprocessing applications, including antibody capture, polishing, and large-scale purification. It supports multiple regeneration cycles with minimal loss in performance and is fully compatible with standard chromatography systems, making it a robust and scalable solution for antibody-based workflows.
 
       
         
      | Properties | |
|---|---|
| Matrix | Crosslinked agarose beads | 
| Ligand | High molecular weight Dextran sulfate | 
| Particle diameter (µm) | 30–165 µm | 
| Lysozyme binding capacity | ≥20 mg/mL resin | 
| Sulphur content | 50 µM/mL | 
| Recommended CIP solution | 0.2(M) NaOH, 0.2(M) NaOH in 20% EtOH, 0.2(M) NaOH in 95% EtOH | 
| pH stability range | 1–13 | 
| Storage | 2–8 °C in 20% ethanol | 
| Characteristics of Protein Recovery | Possible to enter the pore but low adsorption and high recovery under salt addition conditions | 
Protein A resin is a versatile tool used in various protein purification applications:
High Affinity and Selectivity: Offers strong and specific binding for antibodies, minimizing non-specific interactions with other proteins.
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