GeloPure DEAE is a weak anion Exchanger chromatography media based on spherical particles manufactured from crosslinked agarose. Each offers excellent flow properties, mechanical stability and chemical resistance. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range, pH 2 to 10. These ion exchangers are ideally suited for both laboratory and process scale chromatography of proteins, peptides, and other biomolecules.
Agarose a natural polysaccharide with a unique structure. This gives agarose special pore structure, which plays an important role in chromatography. GeloPure DEAE, made from crosslinked agarose 4%, benefits from this structure by offering good mechanical strength and efficient flow of large biomolecules. This helps improve performance when purifying large proteins and other biological substances.
GeloPure DEAE is made from strong, cross-linked spherical agarose with excellent flow characteristics and high binding capacity. It allows flow rates of 300 to 400 cm/h through a 15 cm column at 1 bar (14.5 psi, 0.1 MPa), enabling quick separation steps—especially useful in the early stages of purification when fast processing is important. During washing and equilibration, the flow rate can be increased up to 750 cm/h for even faster operation. The basic characteristics of GeloPure DEAE weak anion exchange chromatography media are shown in Table 1.
| Attribute | CelloPure DEAE |
|---|---|
| Ion Exchange Type | Weak anion exchange |
| Ligand Name | DEAE (diethylaminoethyl) |
| Base Matrix | Cross-linked agarose beads 4% |
| Particle Size (μm) | 30 μm to 165 μm |
| pH Working Range | 2–12 |
| Ion Exchange Capacity (meq/ml-gel) | 0.08 to 0.20 |
| Operating Pressure | Up to 2 bar (0.2 MPa) |
| Dynamic Binding Capacity (mg/ml) | HSA – 36 Human Immunoglobulin – 18 |
| Supplied | Suspension in 20% Ethanol |
Accommodates larger sample loads and improves resolution for more efficient purification.
Functions effectively across a broader pH spectrum, offering greater flexibility in purification protocols.
Built on a robust agarose or synthetic polymer matrix, ensuring enhanced stability compared to cellulose-based resins.
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