CelloPure DEAE

Ion Exchange Chromatography Media

CelloPure DEAE

CelloPure DEAE is a weak anion exchanger chromatography media based on spherical particles manufactured from highly cross-linked cellulose. Each offers excellent flow properties, mechanical stability and chemical resistance. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range, pH 2 to 12. These ion exchangers are ideally suited for both laboratory and process scale chromatography of proteins, peptides and other biomolecules.

CelloPure DEAE

CelloPure DEAE base resin

Cellulose is a natural polysaccharide with a unique crystalline structure, unlike non-crystalline polysaccharides like agarose. This gives cellulose a special pore structure, which plays an important role in chromatography. CelloPure DEAE, made from crosslinked cellulose, benefits from this structure by offering good mechanical strength and efficient flow of large biomolecules. This helps improve performance when purifying large proteins and other biological substances.

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Pressure-flow and Characteristics of CelloPure DEAE

CelloPure DEAE is made from strong, cross-linked spherical cellulose with excellent flow characteristics and high binding capacity. It allows flow rates of 300 to 500 cm/h at 1 bar (14.5 psi, 0.1 MPa), enabling quick separation steps—especially useful in the early stages of purification when fast processing is important. During washing and equilibration, the flow rate can be increased up to 750 cm/h for even faster operation. The basic characteristics of CelloPure DEAE weak anion exchange chromatography media are shown in Table 1.

cellopure_deae_graph
  • Column : HiScale 16/20, 5 cm bed height, 10 ml bed volume
  • Flow rate : 1ml/min
  • Sample : 20mg/ml
  • Start Buffer : 30mM Phosphate buffer (pH 7.8-8.2) for HSA, 50mM Tris-HCL (pH 9.5) for IgG
  • Elution Buffer : 30mM Sodium Acetate (pH 4.6) for HSA, 50mM Tris-HCL+1M Nacl (pH 9.5) for IgG
CelloPure DEAE 100 mL Pack
CelloPure DEAE Resin, 50 mL
High-performance resin for efficient biomolecule separation.
PT-DEAE-50ML
On Request
CelloPure DEAE Resin, 100 mL
High-performance resin for efficient biomolecule separation.
PT-DEAE-100ML
On Request
Pack Size
100 mL
50 mL

Table. Basic characteristics of CelloPure DEAE weak anion exchange chromatography media

Attribute CelloPure DEAE Resin
Ion Exchange Type Weak anion exchange
Functional Group DEAE (di-ethylaminoethyl)
Base Matrix Cross-linked Cellulose Beads
Exclusion Limit (Da) 360,000
Particle Size (µm) 40–130 µm
pH Working Range 2 to 12
Operating Pressure Up to 2 bar (0.2 Mpa)
Ion Exchange Capacity (meq/ml-gel) 0.08 to 0.20
Dynamic Binding Capacity (mg/ml) BSA: >45
Human IgG: 20
Supplied Suspension in 20% Ethanol

Advantages

Higher Binding Capacity

Accommodates larger sample loads and improves resolution for more efficient purification.

Wider pH Range

Functions effectively across a broader pH spectrum, offering greater flexibility in purification protocols.

Superior Chemical Stability

Built on a robust agarose or synthetic polymer matrix, ensuring enhanced stability compared to cellulose-based resins.

Achieve greater efficiency and flexibility in your purification processes with CelloPure DEAE Resin