CelloPure DEAE is a weak anion exchanger chromatography media based on spherical particles manufactured from highly cross-linked cellulose. Each offers excellent flow properties, mechanical stability and chemical resistance. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range, pH 2 to 12. These ion exchangers are ideally suited for both laboratory and process scale chromatography of proteins, peptides and other biomolecules.
Cellulose is a natural polysaccharide with a unique crystalline structure, unlike non-crystalline polysaccharides like agarose. This gives cellulose a special pore structure, which plays an important role in chromatography. CelloPure DEAE, made from crosslinked cellulose, benefits from this structure by offering good mechanical strength and efficient flow of large biomolecules. This helps improve performance when purifying large proteins and other biological substances.
CelloPure DEAE is made from strong, cross-linked spherical cellulose with excellent flow characteristics and high binding capacity. It allows flow rates of 300 to 500 cm/h at 1 bar (14.5 psi, 0.1 MPa), enabling quick separation steps—especially useful in the early stages of purification when fast processing is important. During washing and equilibration, the flow rate can be increased up to 750 cm/h for even faster operation. The basic characteristics of CelloPure DEAE weak anion exchange chromatography media are shown in Table 1.
| Attribute | CelloPure DEAE Resin |
|---|---|
| Ion Exchange Type | Weak anion exchange |
| Functional Group | DEAE (di-ethylaminoethyl) |
| Base Matrix | Cross-linked Cellulose Beads |
| Exclusion Limit (Da) | 360,000 |
| Particle Size (µm) | 40–130 µm |
| pH Working Range | 2 to 12 |
| Operating Pressure | Up to 2 bar (0.2 Mpa) |
| Ion Exchange Capacity (meq/ml-gel) | 0.08 to 0.20 |
| Dynamic Binding Capacity (mg/ml) | BSA: >45 Human IgG: 20 |
| Supplied | Suspension in 20% Ethanol |
Accommodates larger sample loads and improves resolution for more efficient purification.
Functions effectively across a broader pH spectrum, offering greater flexibility in purification protocols.
Built on a robust agarose or synthetic polymer matrix, ensuring enhanced stability compared to cellulose-based resins.
Copyright © 2025 BIOTRUM, All Rights Reserved.
Subscribe for daily latest news and updates
Copyright © 2025 BIOTRUM, All Rights Reserved.
BioNutra Inc. leads the healthcare industry with innovative, science-driven nutraceuticals, combining quality, affordability, and sustainability to inspire healthier living worldwide.
Copyright © 2025 BIOTRUM, All Rights Reserved.
Automated page speed optimizations for fast site performance