GeloPure SP

Ion Exchange Chromatography Media

GeloPure SP

Introduction

GeloPure SP is a strong cation exchanger chromatography media based on spherical particles manufactured from crosslinked Agarose. Each offers excellent flow properties, mechanical stability, and chemical resistance. In this ion exchange resin, the sulphopropyl (SP) functional group is attached to the cross-linked agarose beads, which remains charged and maintains consistently high capacity over the entire working range, pH 3 to 11. These ion exchangers are ideally suited for both laboratory and process scale chromatography of proteins, peptides, and other biomolecules.

gelopure_sp

Optical micrograph of GeloPure SP Resin

GeloPure SP base resin

Cross-linked spherical agarose 4% beads are prepared by unique process from natural polysaccharides. Primarily composed of agarobiose repeating units (alternating D-galactose and 3,6-anhydro-L-galactose), finally beads are highly hydrophilic, minimize the non-specific binding. GeloPure SP, made from cross-linked agarose beads, benefits from this structure by offering efficient flow velocity for large biomolecules. This helps improve performance when purifying large proteins and other biological substances.

Dynamic Binding Capacities of GeloPure SP

GeloPure SP has high efficiency in mass transfer and excellent Dynamic Binding Capacities, particularly for large biomolecules like human Immunoglobulins (IgG) also Lysozyme.

Because of these special qualities, GeloPure SP media can be used in downstream processes in the purification of biopharmaceuticals. With good reliability, protein separation has been scaled up using GeloPure SP from laboratory size and no discernible variations were seen in the purity of single peak at the time of protein separation.

micrograph_sp
  • Column : HiScale 16/20, 5 cm bed height, 10 ml bed volume
  • Flow rate : 1ml/min
  • Sample : 7mg/ml
  • Start Buffer : 0.25M Sodium Acetate (pH 5.6) for IgG, 50mM Tris-HCL + 150 mM Nacl (pH 9.5) for Lysozyme
  • Elution Buffer : 0.25M Sodium Acetate + 0.5M Nacl (pH 5.6) for IgG 50mM Tris-HCL + 1M Nacl (pH 9.5) for Lysozyme
GeloPure SP Resin 100 mL
GeloPure SP Resin, 100 mL
High-performance resin for efficient biomolecule separation.
GP-SP-100ML
On Request
GeloPure SP Resin, 50 mL
High-performance resin for efficient biomolecule separation.
GP-SP-50ML
On Request
Pack Size
100 mL
50 mL

Table 1. Basic characteristics of GeloPure SP strong cation exchange chromatography media

Attribute GeloPure SP Resin
Ion Exchange Type (Functional Group) Strong Cation Exchange, Sulphopropyl group (CH₂CH₂CH₂SO₃)
Base Matrix Cross-linked Cellulose Beads 4%
Particle Size (μm) 30-165 µm
Exclusion Limit (Da) 360,000
pH Working Range 2-12
Operating Pressure Up to 2 bar (0.2 MPa)
Ion Exchange Capacity (meq/ml-gel) 0.11-0.20
Binding Capacity (mg/mL) Human IgG ≥50
Lysozyme >90
Supplied Suspension in 20% Ethanol