CelloPure⁺ DEAE

Ion Exchange Chromatography Media

CelloPure⁺ DEAE

The CelloPure⁺ DEAE weak anion exchanger is a surface-modified, highly crosslinked medium with dextran scaffold structure. It delivers high dynamic binding capabilities and maintains stability even at elevated flow rates. The optimized media's excellent performance presents a substantial chance to boost the throughput of downstream purification. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range, pH 1 to

CelloPure⁺ DEAE base resin

Cellulose is a natural polysaccharide with a unique crystalline structure, unlike non-crystalline polysaccharides like agarose. This gives cellulose a special pore structure, which plays an important role in chromatography. CelloPure⁺ DEAE, made from crosslinked cellulose and scaffold with dextran, benefits from this structure by offering good mechanical strength and efficient flow of large biomolecules. This helps improve performance when purifying large proteins and other biological substances even with high flow rates.

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Pressure-flow and Characteristics of CelloPure⁺ DEAE

CelloPure⁺ DEAE media consist of highly cross-linked cellulose beads with an average diameter of 100 μm, which are surface modified with dextran to enhance their performance. It gives high binding capacities even in high flowrates. It allows flow rates of 400 to 500 cm/h through a 15 cm column at 1 bar (14.5 psi, 0.1 MPa), enabling quick separation steps—especially useful in the early stages of purification when fast processing is important. During washing and equilibration, the flow rate can be increased up to 750 cm/h for even faster operation. The basic characteristics of CelloPure⁺ DEAE weak anion exchange chromatography media are shown in Table 1.

cellopure_deae_graph
  • Column : HiScale 16/20, 5 cm bed height, 10 ml bed volume
  • Flow rate : 1ml/min
  • Sample : 20mg/ml
  • Start Buffer : 30mM Phosphate buffer (pH 7.8-8.2) for HSA, 50mM Tris-HCL (pH 9.5) for IgG
  • Elution Buffer : 30mM Sodium Acetate (pH 4.6) for HSA, 50mM Tris-HCL+1M Nacl (pH 9.5) for IgG
CelloPure⁺ DEAE 100 mL Pack
CelloPure⁺ DEAE Resin, 50 mL
High-performance resin for efficient biomolecule separation.
CP-DEAE-50ML
On Request
CelloPure⁺ DEAE Resin, 100 mL
High-performance resin for efficient biomolecule separation.
CP-DEAE-100ML
On Request
Pack Size
100 mL
50 mL

Table 1. Basic characteristics of CelloPure⁺ DEAE weak anion exchange chromatography media

Weak Anion Exchange CelloPure⁺ DEAE
Ion Exchange Type Weak anion
Functional Group DEAE (di-ethyl aminoethyl group)
Base Matrix Highly Cross-linked Cellulose Beads with Dextran Scaffold
Particle Size (µm) 40–130 µm
Average Size 90 µm
pH Working Range 1 to 12
Operating Pressure Up to 2 Bar (0.2 Mpa)
Ion Exchange Capacity (meq/ml-gel) 0.08 to 0.22
Chemical Stability 0.5N NaOH
Dynamic Binding Capacity (mg/ml) HSA: >100
Human IgG: >30
Supplied Suspension in 20% Ethanol

Advantages

Higher Binding Capacity

Accommodates larger sample loads and improves resolution for more efficient purification.

Wider pH Range

Functions effectively across a broader pH spectrum, offering greater flexibility in purification protocols.

Superior Chemical Stability

Built on a robust agarose or synthetic polymer matrix, ensuring enhanced stability compared to cellulose-based resins.

Achieve greater efficiency and flexibility in your purification processes with CelloPure⁺ DEAE Resin!