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GeloPure A⁺

Affinity Chromatography Media

GeloPure A⁺

Introduction

GeloPure A⁺ resin is basically rProtein A immobilised agaose basedanaffinitychromatography media used for the purification of immunoglobulins (IgG), primarilymonoclonal antibodies. The ligand, recombinant Protein A or native ProteinA, isimmobilized on a spherical crosslinked agarose resin for high binding capacity

GeloPure_pro_A
GeloPure Pro A Resin 100 mL
GeloPure Pro A Resin100 mL GeloPure Pro A Resin 50 mL
GeloPure A⁺ Resin, 100 mL
High-performance resin for efficient biomolecule separation.
GP-A-100ML
On Request
GeloPure A⁺ Resin, 50 mL
High-performance resin for efficient biomolecule separation.
GP-A-50ML
On Request
Pack Size
100 mL
50 mL
GeloPure A⁺ Characteristics
Ligand Recombinant Protein A
Matrix Crosslinked agarose beads
Particle Diameter (µm) 30–165 µm
Ligand Coupling Covalent coupling via multipoint attachment
Dynamic Binding Capacity >65 mg/mL
Recommended CIP Solution 0.2(M) NaOH, 0.2(M) NaOH in 20% EtOH,
0.2(M) NaOH in 95% EtOH
pH Stability Range 1 ~ 13
Storage 2 ~ 8 °C in 20% ethanol
Shelf Life Typically 2 years unopened
Technical Datasheet Coming Soon!

Applications

Protein A resin is a versatile tool used in various protein purification applications:

  • Monoclonal Antibody Purification: The primary application is isolating monoclonal antibodies (mAbs) produced by hybridoma cells or recombinant expression systems.
  • Immunoglobulin Purification: Can be used to purify polyclonal antibodies from serum or other biological fluids.
  • Antibody Fragment Purification: Applicable for isolating antibody fragments like Fab or Fc fragments.
  • Downstream Processing: Can be employed in downstream processing steps of antibody production for purification and removal of contaminants.

Advantages

High Affinity and Selectivity: Offers strong and specific binding for antibodies, minimizing non-specific interactions with other proteins.

  • Easy Operation: Relatively simple to use with standard chromatography techniques.
  • Wide Range of Applications:Suitable for purifying antibodies from various sources and formats.
  • Mild Elution Conditions: Antibodies can be eluted using mild buffers, minimizing potential denaturation.

Products

  • Weak Ion Exchange
  • Strong Ion Exchange
  • Affinity chromatographic Resins
  • Hydrophobic Resins
  • Chromatographic columns
  • Chromatographic systems
    (Under Development)

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