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Recombinant Human TNFα Protein

Recombinant Human TNFα Protein

(Lyophilized, E.Coli coli-derived)

Catalog No: BTC-TNFA-50 | Size: 50 µg

Overview

Our GMP grade Recombinant Human Tumor Necrosis Factor-alpha (TNFα) is a potent pro-inflammatory cytokine primarily produced by activated macrophages and T cells. It plays a pivotal role in systemic inflammation, immune regulation, and apoptosis.

human_TNF

Specifications

  • Expression System: Escherichia coli
  • Purity: ≥98% (SDS-PAGE with Coomassie staining)
  • Molecular Weight: ~17 kDa (monomer) | Active form: homodimer ~34 kDa
  • Specific Activity: Validated by STAT1 phosphorylation in THP-1 cells
  • Endotoxin Level: <0.1 ng/µg (LAL assay)
  • Residual Host Cell DNA: <10 ng/mg (validated qPCR method)
  • Sterility: Free from mycoplasma, bacterial and viral contamination.
  • Formulation: Lyophilized powder, carrier-free
  • Reconstitution: Recommended in sterile water or 0.1% PBS
  • Storage: Store at 2-8°C (lyophilized) and –20°C after reconstitution.
  • Shelf Life: 24 months.

Use: Validated under GMP conditions for use in preclinical and clinical research.

Role:

TNFα is a key cytokine in host defense and immune surveillancE.Coli It regulates immune cell activity, promotes apoptosis in tumor and infected cells, and amplifies inflammatory responses.

Applications

  • Macrophage activation and polarization assays
  • Th1-type immune stimulation studies
  • Cytokine synergy and signaling pathway analysis
  • NK and T-cell functional assays
  • Immunomodulation research in autoimmunity, cancer, and infection
  • Standard control in immunoassays (ELISA, Western blot, etc.)

Highlihgts

  • High biological activity validated in functional cell-based assays
  • Endotoxin controlled for immune-sensitive in vitro studies
  • Lyophilized with stabilizers for improved reconstitution and shelf-life
  • Free from animal-origin components; ideal for translational R&D
  • Scalable production for preclinical to bulk supply requirements
  • Complies with USP (1043)
Available Sizes
Contact
10 µg | 25 µg | 50 µg | 100 µg | Bulk quantities
info@biotrum.co for COA, datasheets, technical queries, or bulk pricing

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