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Recombinant Protein L (E.coli Derived)

Recombinant Protein L

(E.Coli coli Derived)

Catalog No: BTP-ProteinL-1000 | Size: 1000ug / vial | Expression Host: E.Coli coli

Overview

Our GMP grade Recombinant Protein L is a versatile antibody-binding reagent engineered to be produced in E.Coli coli for high purity and reproducibility.

rProtein_L

Relevance in Cell & Gene Therapy

  • Protein L plays a crucial role in the development and quality control of antibody-derived reagents used in advanced therapy medicinal products. In CAR-T and CAR-NK workflows, it is used to:
  • Detect and quantify scFv or Fab regions expressed on genetically modified immune cells
  • Purify engineered antibody fragments from culture media
  • Enable selection and enrichment of transduced cells based on scFv expression
  • Assist in quality control assays to ensure vector expression and functional validation
  • Support screening of synthetic antibody libraries used in TCR/CAR discovery
  • Its ability to bind without affecting the antigen recognition site makes it compatible with downstream functional testing, such as cytotoxicity assays and cytokine profiling.

Specifications

  • Molecular Weight: ~36 kDa
  • Binding Target: Kappa light chains (VL domain)
  • Compatible Fragments: Fab, scFv, diabodies, VHH/nanobodies
  • Expression System: E.Coli coli
  • Purity: ≥95% (SDS-PAGE)
  • Endotoxin Level: <1 EU/µg (LAL assay)
  • Residual Host Cell DNA: <10 ng/mg (validated qPCR method)
  • Sterility: Free from mycoplasma, bacterial and viral contamination.
  • Formulation: Lyophilized powder, carrier-free
  • Reconstitution: Recommended in sterile water or 0.1% PBS
  • Storage: Store at 2-8°C (lyophilized) and –20°C after reconstitution.
  • Shelf Life: 24 months.
  • Use: Validated under GMP conditions for use in preclinical and clinical research.

Applications

  • CAR-T and CAR-NK product characterization
  • Purification of scFv fragments and nanobodies
  • QC of viral vectors expressing antibody-based domains
  • Monitoring transduction efficiency in engineered cells
  • Preclinical screening of antibody libraries for gene-modified therapies

Highlihgts

  • Binds a broad range of antibody fragments without Fc dependency
  • Compatible with both soluble proteins and cell-bound formats
  • Enables high-sensitivity detection of CAR constructs
  • Recombinant, non-glycosylated, endotoxin-controlled
  • Suitable for both preclinical and translational R&D environments
  • Complies with USP (1043)
Available Sizes
Contact
1 mg | 5 mg | 10 mg (custom quantities available)
info@biotrum.co for technical datasheets, magnetic bead conjugates, or OEM/custom bulk inquiries

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