The CelloPureāŗ DEAE weak anion exchanger is a surface-modified, highly crosslinked medium with dextran scaffold structure. It delivers high dynamic binding capabilities and maintains stability even at elevated flow rates. The optimized media's excellent performance presents a substantial chance to boost the throughput of downstream purification. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range, pH 1 to
Cellulose is a natural polysaccharide with a unique crystalline structure, unlike non-crystalline polysaccharides like agarose. This gives cellulose a special pore structure, which plays an important role in chromatography. CelloPureāŗ DEAE, made from crosslinked cellulose and scaffold with dextran, benefits from this structure by offering good mechanical strength and efficient flow of large biomolecules. This helps improve performance when purifying large proteins and other biological substances even with high flow rates.
CelloPureāŗ DEAE media consist of highly cross-linked cellulose beads with an average diameter of 100 μm, which are surface modified with dextran to enhance their performance. It gives high binding capacities even in high flowrates. It allows flow rates of 400 to 500 cm/h through a 15 cm column at 1 bar (14.5 psi, 0.1 MPa), enabling quick separation stepsāespecially useful in the early stages of purification when fast processing is important. During washing and equilibration, the flow rate can be increased up to 750 cm/h for even faster operation. The basic characteristics of CelloPureāŗ DEAE weak anion exchange chromatography media are shown in Table 1.
| Weak Anion Exchange | CelloPureāŗ DEAE |
|---|---|
| Ion Exchange Type | Weak anion |
| Functional Group | DEAE (di-ethyl aminoethyl group) |
| Base Matrix | Highly Cross-linked Cellulose Beads with Dextran Scaffold |
| Particle Size (µm) | 40ā130 µm |
| Average Size | 90 µm |
| pH Working Range | 1 to 12 |
| Operating Pressure | Up to 2 Bar (0.2 Mpa) |
| Ion Exchange Capacity (meq/ml-gel) | 0.08 to 0.22 |
| Chemical Stability | 0.5N NaOH |
| Dynamic Binding Capacity (mg/ml) | HSA: >100 Human IgG: >30 |
| Supplied | Suspension in 20% Ethanol |
Accommodates larger sample loads and improves resolution for more efficient purification.
Functions effectively across a broader pH spectrum, offering greater flexibility in purification protocols.
Built on a robust agarose or synthetic polymer matrix, ensuring enhanced stability compared to cellulose-based resins.
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