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CelloPure ET

Affinity Chromatography Media

CelloPure ET resin is poly(ε-lysine) immobilized cross-linked cellulose beads.

Introduction

The CelloPure ET affinity chromatography media is poly ε-lysine immobilized on crosslinked cellulose beads. The beads bind and remove endotoxin from solution. The poly(ε-lysine) is a microbial poly amino acid that consist of 30-35 lysine residues produced by Streptomyces albulus. The poly ε-lysine as ligand and the crosslink cellulose beads acts as a matrix.

et_resin

CelloPure ET Resin

The CelloPure ET resin is endotoxin removing beads, were developed by Promea therapeutics. The poly(ε-lysine) was immobilized onto epichlorohydrinactivated cellulose beads. The CelloPure ET is a stable affinity beads that are resistant against the clean-up solutions, which include 0.2 M sodium hydroxide and 2 M sodium chloride.

et_resin_optical_micrograph

Optical micrograph of CelloPure ET resin beads

CelloPure ET Resin 100 mL
100 mL 50 mL
CelloPure ET Resin, 100 mL
High-performance resin for efficient biomolecule separation.
CP-ET-100ML
On Request
CelloPure ET Resin, 50 mL
High-performance resin for efficient biomolecule separation.
CP-ET-50ML
On Request
Pack Size
100 mL
50 mL

The basic characteristics of CelloPure ET resin are shown in table 1

Properties
Ligand Poly ε–lysine (n=30–35)
Matrix Crosslinked cellulose beads
Particle diameter (mm) 40–130 µm (90 µm in average)
MW exclusion limit (Mlim) 2×106
Operating Pressure Up to 2 bar (0.2 Mpa)
Recommended SIP solutions 0.2(M) NaOH, 0.2(M) NaOH in 20% EtOH, 0.2(M) NaOH in 95% EtOH
pH stability range 1～13
Storage 2～8 ºC in 20% ethanol
Characteristics of Protein Recovery Possible to enter in the pore but low adsorption and high recovery under salt addition conditions

Table 2: Endotoxin adsorption and protein recovery from two different protein solution

Sample Solution CelloPure ET (100 mM phosphate buffer, pH 7.14)
Sample IVIG
Conc. of Endotoxin Before Treatment (pg/mL) <25,600
Conc. of Endotoxin After Treatment (pg/mL) <10
Recovery of Protein After Treatment (%) 90%
Sample HSA
Conc. of Endotoxin Before Treatment (pg/mL) <12,800
Conc. of Endotoxin After Treatment (pg/mL) <20
Recovery of Protein After Treatment (%) 90%
Technical Datasheet

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