CelloPure Phenyl resin is a chromatography media, can remove aggregates efficiently from antibodies, synthesized by standard polysaccharide ligand modification technology. After affinity chromatography like protein A, protein L or protein G, the antibody samples are passed through CelloPure Phenyl chromatography, the aggregates are bound to the hydrophobic ligand of the chromatography media and antibody monomers are coming out in flowthrough fraction. CelloPure Phenyl can be used as flow-through purification mode that selectively bounds such as aggregates or polymers.
Fig 1. OPM analysis of CelloPure Phenyl resin
Crosslinked spherical Cellulose beads are prepared by unique process from crystalline natural polysaccharides, differing the physical characteristics from noncrystalline polysaccharides such as agarose. Thus, CelloPure resins are highly porous in nature (Fig. 1). Due to that porous nature of the highly crosslinked spherical cellulose bead, resins have unique mechanical strength.
Partial structure of CelloPure Phenyl
Ligand structure for CelloPure Phenyl media is described.
CelloPure Phenyl enable high-flow operations, for efficient purification in biopharmaceutical industries high flow operation is essential. The figures below show pressure-flow velocity curves of CelloPure Phenyl (Fig. 3). CelloPure Phenyl is operable at practical flow velocities and pressures.
CelloPure Phenyl have high efficiency in mass transfer and excellent Dynamic Binding Capacities, particularly for large biomolecules like Immunoglobulins (IgG) also for HSA and Ribonuclease.
Because of these special qualities, CelloPure CM media can be used in downstream processes in the purification of biopharmaceuticals.
| Characteristics | |
|---|---|
| Ligand | Phenyl group |
| Type | Hydrophobic |
| Base Matrix | Spherical, Highly Cross-linked Cellulose Beads |
| Particle Size (µm) | 40 - 130 µm (Avg: 90 µm) |
| pH Working Range | 2 to 14 |
| Operating Pressure | Up to 2 bar (0.2 Mpa) |
| Chemical Stability | 0.5N NaOH |
| Binding Capacity (mg/mL resin) | HNA*: 44 Human-IgG**: 18 |
| Supplied | Suspension in 20% Ethanol |
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