CelloPure Q

Ion Exchange Chromatography Media

CelloPure Q

CelloPure Q is a strong anion exchanger chromatography media, surface-modified highly cross-linked cellulose medium. It delivers high dynamic binding capabilities and maintains stability even at elevated flow rates. The optimized media's excellent performance presents a substantial chance to boost the throughput of downstream purification. In this ion exchange resin, the Quaternary amine functional group is attached to the cellulose beads, which remains charged and maintains consistently high capacity over the entire working range, pH 2 to 12.

CelloPure Q base resin

Cellulose is a natural polysaccharide with a unique crystalline structure, unlike noncrystalline polysaccharides like agarose. This gives cellulose a special pore structure, which plays an important role in chromatography. CelloPure Q, made from crosslinked cellulose benefits from this structure by offering good mechanical strength and efficient flow of large biomolecules. This helps improve performance when purifying large proteins and other biological substances even with high flow rates.

Dynamic Binding Capacities of CelloPure Q

CelloPure Q have high efficiency in mass transfer and excellent Dynamic Binding Capacities, particularly for HSA), and large biomolecules like Immunoglobulins (IgG).

Because of these special qualities, CelloPure Q media can be used in downstream processes in the purification of biopharmaceuticals. With good reliability, protein separation has been scaled up using CelloPure Q from laboratory size and no discernible variations were seen in the purity of single peak at the time of protein separation.

CelloPure_Q_binding_modules
  • Column : HiScale 16/20, 5 cm bed height, 10 ml bed volume
  • Flow rate : 1ml/min
  • Sample : 20mg/ml
  • Start Buffer : 30mM Phosphate buffer (pH 7.8-8.2) for HNA, 50mM Tris-HCL (pH 9.5) for IgG
  • Elution Buffer : 30mM Sodium Acetate (pH 4.6) for HNA, 50mM Tris-HCL+1M Nacl (pH 9.5) for IgG
CelloPure Q Resin 100 mL Pack
CelloPure Q Resin, 50 mL
High-performance resin for efficient biomolecule separation.
CP-Q-50ML
On Request
CelloPure Q Resin, 100 mL
High-performance resin for efficient biomolecule separation.
CP-Q-100ML
On Request
Pack Size
100 mL
50 mL

Table 1. Basic characteristics of CelloPure Q strong anion exchange chromatography media

Attribute CelloPure Q
Type Strong anion exchange
Ligand -N+(CH3)3
Base Matrix Highly Cross-linked Cellulose Beads
Particle Size (μm) 40–130 μm
Exclusion Limit (Da) 360,000
pH Working Range 2–11
Operating Pressure Up to 2 bar (0.2 MPa)
Ion Exchange Capacity (meq/ml-gel) 0.1–0.24
Chemical Stability 0.5N NaOH
Dynamic Binding Capacity (mg/ml) HSA >45
Human IgG – 35
Supplied Suspension in 20% Ethanol