CelloPure SP

Ion Exchange Chromatography Media

CelloPure SP

Introduction

CelloPure SP is a strong cation exchanger chromatography media based on spherical particles manufactured from highly crosslinked cellulose. Each offers excellent flow properties, mechanical stability, and chemical resistance. In this ion exchange resin, the sulphopropyl (SP) functional group is attached to the cellulose beads, which remains charged and maintains consistently high capacity over the entire working range, pH 3 to 11. These ion exchangers are ideally suited for both laboratory and process scale chromatography of proteins, peptides, and other biomolecules.

cellopure_sp

Optical micrograph of CelloPure SP Resin

CelloPure SP base resin

Cellulose is a natural polysaccharide with a unique crystalline structure, unlike non-crystalline polysaccharides like agarose. This gives cellulose a special pore structure, which plays an important role in chromatography. CelloPure SP, made from crosslinked cellulose, benefits from this structure by offering good mechanical strength and efficient flow of large biomolecules. This helps improve performance when purifying large proteins and other biological substances.

Dynamic Binding Capacities of CelloPure SP

CelloPure SP have high efficiency in mass transfer and excellent Dynamic Binding Capacities, particularly for large biomolecules like Immunoglobulins (IgG) also for HNA (Human Normal Albumin) and Lysozyme, Ribonuclease.

Because of these special qualities, CelloPure SP media can be used in downstream processes in the purification of biopharmaceuticals. With good reliability, protein separation has been scaled up using CelloPure SP from laboratory size and no discernible variations were seen in the purity of single peak at the time of protein separation.

micrograph_sp
  • Column : HiScale 16/20, 5 cm bed height, 10 ml bed volume
  • Flow rate : 1ml/min
  • Sample : 7mg/ml
  • Start Buffer : 0.25M Sodium Acetate (pH 5.6) for IgG, 50mM Tris-HCL + 150 mM Nacl (pH 9.5) for Lysozyme
  • Elution Buffer : 0.25M Sodium Acetate + 0.5M Nacl (pH 5.6) for IgG, 50mM Tris-HCL + 1M Nacl (pH 9.5) for Lysozyme
CelloPure SP Resin 100 mL
CelloPure SP Resin, 100 mL
High-performance resin for efficient biomolecule separation.
CP-SP-100ML
On Request
CelloPure SP Resin, 50 mL
High-performance resin for efficient biomolecule separation.
CP-SP-50ML
On Request
Pack Size
100 mL
50 mL

Table 1. Basic characteristics of CelloPure SP strong cation exchange chromatography media

Attribute CelloPure SP Resin
Ion Exchange Type (Functional Group) Strong cation exchange, Sulphopropyl group
Base Matrix Cross-linked Cellulose Beads
Particle Size (μm) 40-130 µm
Exclusion Limit (Da) 360,000
pH Working Range 2-12
Operating Pressure Up to 2 bar (0.2 MPa)
Ion Exchange Capacity (meq/ml-gel) 0.11-0.20
Binding Capacity (mg/mL) Human IgG 56
Lysozyme 150
Supplied Suspension in 20% Ethanol