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GeloPure A resin is basically rProtein A immobilised agaose based an affinity chromatography media used for the purification of immunoglobulins (IgG), primarily monoclonal antibodies. The ligand, recombinant Protein A or native Protein A, is immobilized on a spherical crosslinked agarose resin for high binding capacity
Protein A is a cell wall protein originally derived from Staphylococcus aureus. It specifically binds to the Fc region of immunoglobulin G (IgG) molecules, making it ideal for antibody purification.
| GeloPure A – Characteristics | |
|---|---|
| Ligand | Recombinant Protein A |
| Matrix | Crosslinked agarose beads |
| Particle Diameter (µm) | 30–165 µm |
| Ligand Coupling | Covalent coupling via multipoint attachment |
| Dynamic Binding Capacity | >40 mg/mL |
| Recommended CIP Solution | 0.2(M) NaOH, 0.2(M) NaOH in 20% EtOH, 0.2(M) NaOH in 95% EtOH |
| pH Stability Range | 1 ~ 13 |
| Storage | 2 ~ 8 °C in 20% ethanol |
| Shelf Life | Typically 2 years unopened |
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