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GeloPure CM

Ion Exchange Chromatography Media

GeloPure CM Resin

Introduction

GeloPure CM is a weak cation exchanger chromatography media based on spherical particles manufactured from cross-linked agarose. Each offers excellent flow properties and chemical resistance. In this ion exchange resin, the Carboxymethyl (CM) functional group is attached to the agarose spherical beads, which remains charged and maintains consistently high capacity over the entire working range, pH 2 to 11. These ion exchangers are ideally suited for both laboratory and process scale chromatography of proteins, peptides, and other biomolecules.

gelopure_cm

Optical micrograph of GeloPure CM Resin

GeloPure CM base resin

Cross-linked spherical agarose beads are prepared by unique process from natural polysaccharides. Primarily composed of agarobiose repeating units (alternating D-galactose and 3,6-anhydro-L-galactose), finally beads are highly hydrophilic, minimize the non-specific binding. GeloPure CM, made from cross-linked agarose beads, benefits from this structure by offering efficient flow velocity for large biomolecules. This helps improve performance when purifying large proteins and other biological substances.

Dynamic Binding Capacities of GeloPure CM

GeloPure CM have high efficiency in mass transfer and excellent Dynamic Binding Capacities, particularly for large biomolecules like Immunoglobulins (IgG) and Lysozyme. Because of these special qualities, GeloPure CM media can be used in downstream processes in the purification of biopharmaceuticals. With good reliability, protein separation has been scaled up using GeloPure CM from laboratory size and no discernible variations were seen in the purity of single peak at the time of protein separation.

  • Column : HiScale 16, 5 cm bed height, 10 ml bed volume
  • Flow rate : 1ml/min
  • Sample : 7mg/ml
  • Start Buffer : 0.25M Sodium Acetate (pH 5.6) for IgG, 10mM Acetate buffer (pH 3.7) for HNA, 50mM Tris-HCL + 150 mM Nacl (pH 9.5) for Lysozyme
  • Elution Buffer : 0.25M Sodium Acetate + 0.5M Nacl (pH 5.6) for IgG, 10mM Acetate + 1M Nacl (pH 5.5) for HNA, 50mM Tris-HCL + 1M Nacl (pH 9.5) for Lysozyme
GeloPure CM Resin 100 mL
GeloPure CM Resin 100 mL Thumbnail GeloPure CM 50ml Thumbnail
GeloPure CM Resin, 100 mL
High-performance resin for efficient biomolecule separation.
GP-CM-100ML
On Request
GeloPure CM Resin, 50 mL
High-performance resin for efficient biomolecule separation.
GP-CM-50ML
On Request
Pack Size
100 mL
50 mL

Table 1. Basic characteristics of GeloPure CM weak cation exchange chromatography media

Characteristics
Ion Exchange Type (Functional Group) Carboxymethyl group
Base Matrix Spherical, Cross-linked agarose Beads 4%
Particle Size (µm) 30 µm to 165 µm
pH Working Range 2 to 11
Operating Pressure 300 to 500 cm/h at < 0.1 MPa in a HiScale 50/20 column with 5 cm diameter and 15 cm bed height (at 25°C using buffers with the same viscosity as water)
Ion Exchange Capacity (meq / mL-gel) 0.09–0.14
Dynamic Binding Capacity (mg/mL) HSA: NA
Human IgG: 46
Lysozyme: 80
Supplied Suspension in 20% Ethanol
Technical Datasheet

Applications

CelloPure CM, CelloPure+ CM and GeloPure CM finds application in various bioseparation techniques:

  • Protein Purification: Separates proteins based on their net charge at neutral pH. Proteins with a higher positive charge will bind more strongly to CelloPure CM Resin
  • Desalting: Removes salts and other small negatively charged molecules from protein samples.
  • Cation Exchange Chromatography: Can be used in a broader context for isolating and purifying various positively charged biomolecules.

Advantages

  • Mild Operating Conditions: Operates effectively at neutral pH, minimizing potential protein denaturation.
  • Easy Regeneration: Regeneration with low ionic strength buffers or dilute salt solutions is relatively simple.
  • Good Biocompatibility: Cellulose matrix minimizes non-specific interactions with biomolecules.

Products

  • Weak Ion Exchange
  • Strong Ion Exchange
  • Affinity chromatographic Resins
  • Hydrophobic Resins
  • Chromatographic columns
  • Chromatographic systems
    (Under Development)

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