GeloPure Phenyl resin is a chromatography media, can remove aggregates efficiently from antibodies, synthesized by standard polysaccharide ligand modification technology. After affinity chromatography like protein A, protein L or protein G, the antibody samples are passed through GeloPure Phenyl chromatography, the aggregates are bound to the hydrophobic ligand of the chromatography media and antibody monomers are coming out in flowthrough fraction. GeloPure Phenyl can be used as flow-through purification mode that selectively bounds such as aggregates or polymers.
Fig 1. OPM analysis of GeloPure Phenyl resin
Cross-linked spherical agarose beads are prepared by unique process from natural polysaccharides. Primarily composed of agarobiose repeating units (alternating D-galactose and 3,6-anhydro-L-galactose), finally beads are highly hydrophilic, minimize the non-specific binding. GeloPure Phenyl, made from crosslinked agarose beads, benefits from this structure by offering efficient flow velocity for large biomolecules. This helps improve performance when purifying large proteins and other biological substances.
Partial structure of GeloPure Phenyl
GeloPure Phenyl enable high-flow operations, for efficient purification in biopharmaceutical industries high flow operation is essential. The figures below show pressure-flow velocity curves of GeloPure Phenyl. GeloPure Phenyl is operable at practical flow velocities and pressures.
GeloPure Phenyl have high efficiency in mass transfer and excellent Dynamic
Binding Capacities, particularly for large biomolecules like Immunoglobulins (IgG)
also for HSA (Human Normal Albumin) and Ribonuclease.
| Characteristics | |
|---|---|
| Ligand | Phenyl group |
| Type | Hydrophobic |
| Base Matrix | Cross-linked Agarose Beads 4% |
| Particle Size (µm) | 40 µm to 165 µm |
| pH Working Range | 2 to 14 |
| Operating Pressure | Up to 2 bar (0.2 Mpa) |
| Chemical Stability | 0.5N NaOH |
| Binding Capacity (mg/mL resin) |
HNA*: 38 Human-IgG**: 15 |
| Supplied | Suspension in 20% Ethanol |
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