GeloPure Phenyl

Hydrophobic Chromatography Media

GeloPure Phenyl

Introduction

GeloPure Phenyl resin is a chromatography media, can remove aggregates efficiently from antibodies, synthesized by standard polysaccharide ligand modification technology. After affinity chromatography like protein A, protein L or protein G, the antibody samples are passed through GeloPure Phenyl chromatography, the aggregates are bound to the hydrophobic ligand of the chromatography media and antibody monomers are coming out in flowthrough fraction. GeloPure Phenyl can be used as flow-through purification mode that selectively bounds such as aggregates or polymers.

butyl_resin

 Fig 1. OPM analysis of GeloPure Phenyl resin

GeloPure Phenyl base resin

Cross-linked spherical agarose beads are prepared by unique process from natural polysaccharides. Primarily composed of agarobiose repeating units (alternating D-galactose and 3,6-anhydro-L-galactose), finally beads are highly hydrophilic, minimize the non-specific binding. GeloPure Phenyl, made from crosslinked agarose beads, benefits from this structure by offering efficient flow velocity for large biomolecules. This helps improve performance when purifying large proteins and other biological substances.

phenyl_formula

Partial structure of GeloPure Phenyl

Pressure-flow Properties of GeloPure Phenyl

GeloPure Phenyl enable high-flow operations, for efficient purification in biopharmaceutical industries high flow operation is essential. The figures below show pressure-flow velocity curves of GeloPure Phenyl. GeloPure Phenyl is operable at practical flow velocities and pressures.

phenyl_sheet

Dynamic Binding Capacities of GeloPure Phenyl

GeloPure Phenyl have high efficiency in mass transfer and excellent Dynamic
Binding Capacities, particularly for large biomolecules like Immunoglobulins (IgG)
also for HSA (Human Normal Albumin) and Ribonuclease.

graph_cellopure_phenyl
GeloPure Phenyl 100 mL
GeloPure Phenyl, 100 mL
High-performance resin for efficient biomolecule separation.
GP-PHENYL-100ML
On Request
GeloPure Phenyl, 50 mL
High-performance resin for efficient biomolecule separation.
GP-PHENYL-50ML
On Request
Pack Size
100 mL
50 mL

The basic characteristics of GeloPure Phenyl Resin are shown in table 1

Characteristics
Ligand Phenyl group
Type Hydrophobic
Base Matrix Cross-linked Agarose Beads 4%
Particle Size (µm) 40 µm to 165 µm
pH Working Range 2 to 14
Operating Pressure Up to 2 bar (0.2 Mpa)
Chemical Stability 0.5N NaOH
Binding Capacity
(mg/mL resin)
HNA*: 38
Human-IgG**: 15
Supplied Suspension in 20% Ethanol