GeloPure Q strong anion exchanger is a surface-modified, highly cross-linked medium with dextran scaffold structure. It delivers high dynamic binding capabilities and maintains stability even at elevated flow rates. The optimized media's excellent performance presents a substantial chance to boost the throughput of downstream purification. In this ion exchange resin, the Quaternary amine functional group is attached to the Cross-linked agarose beads, which remains charged and maintains consistently high capacity over the entire working range, pH 2 to 12.
Cross-linked spherical agarose beads are prepared by unique process from natural polysaccharides. Primarily composed of agarobiose repeating units (alternating D-galactose and 3,6-anhydro-L-galactose), finally beads are highly hydrophilic, minimize the non-specific binding. GeloPure Q, made from cross-linked agarose 4% beads, benefits from this structure by offering efficient flow velocity for large biomolecules. This helps improve performance when purifying large proteins and other biological substances.
GeloPure Q media consist of highly cross-linked agarose beads. It gives high binding capacities even in high flowrates. It allows flow rates of 600 cm/h through at 1 bar (14.5 psi, 0.1 MPa), enabling quick separation steps—especially useful in the early stages of purification when fast processing is important. During washing and equilibration, the flow rate can be increased up to 750 cm/h for even faster operation. The basic characteristics of GeloPure Q weak anion exchange chromatography media are shown in Table 1.
| Attribute | GeloPure Q |
|---|---|
| Ion Exchange Type | Strong anion exchange |
| Ligand Name | -N+(CH3)3 |
| Base Matrix | Highly Cross-linked agarose 4% |
| Particle Size (μm) | 40 μm to 165 μm |
| pH Working Range | 2 to 12 |
| Operating Pressure | Up to 2 Bar (0.2 Mpa) |
| Ion Exchange Capacity (meq/ml-gel) | 0.10–0.24 |
| Chemical Stability | 0.5N NaOH |
| Dynamic Binding Capacity (mg/ml) | HSA – >100 Human γ-globulin – 35 |
| Supplied | Suspension in 20% Ethanol |
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